In line with the COP22 also held in Morocco in 2016 in which Averda has been proactively involved, ACRIS
3 convened a set of high-level expert panels to showcase priority areas of private sector investment for climate resilient infrastructure in Africa - a fundamental building block for Africa to meet its sustainable development goals.
Six species called across these surveys: Northern Cricket Frogs (Acris
crepitans), Gulf Coast Toads (Incilius nebulifer), Cliff Chirping Frogs (Eleutherodaclylus marnockii), Spotted Chorus Frogs (Pseudacris clarkii), American Bullfrogs (Lithobates calesbeianus), and Rio Grande Leopard Frogs (Lilhobates berlandieri).
Slides were incubated in a humidified chamber for 1 h at room temperature with primary antibody: EGFR (RM-2111-S0, dilution 1:50, Thermo Scientific), p53 (M3629, dilution: 1:100, DAKO GmbH, Hamburg, Germany), (RM-2111-S0, dilution 1:50, Thermo Scientific), c-Myc (MS-139-PCL, dilution 1:50, Thermo Scientific), Ki67 (abl6667, dilution 1:100, Abeam, Cambridge, UK), CD31 (MS353-S0, dilution 1:50, Thermo Scientific), P-glycoprotein (CD243, AM05632PU-N, dilution 1:200, Acris
, Herford, Germany), GSTP1 (AP02100SU-S, Acris
), COX2 (AMU127PU-N, dilution 1:100, Acris
), LOX1 (HPA050798, dilution 1:300, Sigma), TFR (CD71; MS-1096S0, dilution 1:50, Thermo Scientific), and VEGF (Ab-7, clone VG-1, which recognized 121,165, and 189 isoformes of VEGF; dilution 1:150, Thermo Scientific).
Twelve species were observed and recorded: Anayrus americanus, Anaxyrus fowleri, Acris
crepitans, Hyla chrysoscelis, Hyla cinerea, Hyla gratiosa, Psuedacris crucifer, Pseudacris feriarum, Gastrophryne carolinensis, Lithobates catesbeiana, Lithobates clamitans, and Lithobates sphenocephala.