Abbreviations: AO, acridine
orange; EB, ethidium bromide; JC-1, 5,5', 6,6'-tetrachloro-1,1', 3,3'-tetraethylbenzimidazolcarbocyanine iodide; [PSI][[DELTA].sub.m], mitochondrial transmembrane potential; PI, propidium iodide; PS, phosphatidylserine.
orange direct cell counts of the flushed and nonflushed soils decreased over the 15-week testing period, but after 5 weeks, the flushed soils maintained higher cell counts than the nonflushed soils.
orange method showed a significant increase of abnormal metachromatic spermatozoa in treated animals (red fluorescence).
Three commonly used fluorescent stains are auramine-rhodamine, acridine
orange and calcofluor white.
The overall project has been divided into four separate phases: synthesis of the acridine
derivative chosen as the intercalating moiety, synthesis of the bidentate ligands, coupling of the ligands to the acridine
derivative, and chelation of the ligands with bis(bipyridyl)ruthenium(ll)dichloride.
Different techniques have been used to determine the integrity of DNA in which acridine
orange (AO) assay is easy and less costly.
Schneider's insect medium, lipopolysaccharide (LPS), Acridine
Orange, Ethidium Bromide, IFN-[gamma], and hydroxylamine were obtained from SIGMA-Aldrich (St Louis, MO, USA).
Quantitative Buffy Coat (QBC) was done using QBC centrifuge and QBC capillary blood tube at 12000 rotations per minute for 5 minutes using Acridine
Orange stain to highlight the parasite.
Methods: The B16F10 cell line was treated with different concentrations of saponin (O, 4, 8, 12, 16, 20 [micro]g/ml), dacarbazine (O, 1200, 1400, 1600, 1800, 2000 [micro]/ml) and coadministration of saponin-dacarbazine (1200 da+8 sp, 1200 da+4 sp) for 24 and 48 hr and the cytotoxic effect was examined by MTT, DAPI, acridine
orange/propodium iodide, flow cytometry and caspase colorimetric assay.
Briefly, cells were washed with PBS, followed by staining with 1 [micro]g/ml acridine
orange for 15 min.
Coverslips were rinsed with PBS and were stained with acridine
orange for 10 min.
Collagenase (type IV), silver-lactate, bafilomycin A1, acridine
orange (AO), and tetramethylrhodamine methyl ester (TMRM) were purchased from Sigma-Aldrich (USA).