In addition, the fundamentals and laboratory techniques commonly used for sample analysis such as
fluorometry, centrifugation, electrophoresis, photometry, and chromatography have also been covered.
spectrophotometry [7,13,41] and
fluorometry [41], to gas
A heterochromatin study demonstrating the non-linearity of
fluorometry useful for calculating genomic base-composition.
After mixing by pipetting up and down approximately 10 times, a 150-pL aliquot was transferred to the fluorometer plate and submitted to
fluorometry as above.
Recently, the methods for FQ residue analysis in food matrices of animal origin were mainly based on high-performance liquid chromatography with
fluorometry or mass spectrometry and immunoassay [6-15].
The eluted drug was determined by
fluorometry ([[lambda].sub.ex] = 300 nm; [[lambda].sub.em] = 404 nm) [9].
Simultaneous high-performance liquid chromatographic determination of retinol by
fluorometry and of tocopherol by ultraviolet absorbance in the serum of newborns.
All experiments were conducted using this medium except 2'7'-dichlorofluorescein diacetate (DCFHDA)
fluorometry. Differentiation of THP-1 cells was optimised based on previously described protocols using PMA (also termed 12-O-tetradecanoylphorbol-13-acetate or TPA) [33-36].
For the detection of the one-photon fluorescence signal that is usually analyzed in conventional
fluorometry, the 365 nm line of a Hg lamp was additionally prepared as the excitation light source.
After incubation and washing, the amount of the bound europium-labelled antibody was assessed by time-resolved
fluorometry (Delphia; Wallac, Turku, Finland).
These include chromatographic methods [5-9],
fluorometry [10-12], flow injection analysis [13,14], atomic absorption [15] and electroanalytical methods [16].