The microscopic photo documentation was made bya Leica DM 2000 photomicroscope
. The embryos at the start of development were blocked whole.
Pancreas sections were stained with hematoxilin and eosin (H&E) then slides were observed under a Leica photomicroscope
The liver sections (5 [micro]m) were examined with a photomicroscope
after staining with hematoxylin and eosin dye (H&E).
All sections were examined and photographed using a light photomicroscope
(Nikon Eclipse E600; USA).
All samples were analyzed and photographed with a Leica[R] DM500 photomicroscope
coupled to a Leica[R] ICC50HD camera.
Several sections were chosen for photomicrography using Zeiss photomicroscope
supplied by Canon digital camera to describe the different developmental changes of the middle ear and its relation to the different neighboring structures.
A Nikon Eclipse 80i photomicroscope
with 200 x magnification was used to obtain the images, which were then analyzed with the Image Pro Plus 6.0 software (Media Cybernetics, USA).
For general histopathological evaluation, tissue sections of 4 [micro]m were stained with hematoxylin and eosin (H&E), and examined under a photomicroscope
(Olympus BX51, Tokyo, Japan) by an experienced histologist who was unaware of the treatment conditions.
Slides were observed with an Olympus BH 2 light photomicroscope
in immersion 1000 X.
The detailed cross-sections were visualized by Motic[R] BA 210 photomicroscope
coupled to Moticam 3MP camera and the longitudinal and transverse sections using Leica EZ4HD Lens.
The analysis and photographic documentation were performed with a Nikon Eclipse-50 photomicroscope
Dispersions of celluloses in water were observed using a photomicroscope