The Centre d'Infectiologie Charles Merieux du Mali in Bamako performed conventional reverse transcription
PCR and gel electrophoresis on the LASV.
Differential display reverse transcription
PCR (DDRT-PCR), introduced firstly by Liang and Pardee (Liang and Pardee, 1992), can identify the total number of genes subject to up- or down-regulation in a particular setting and has been widely used to screen for genes that are differentially expressed (Breyne and Zabeau, 2001; Ahmed et al., 2000).
RNA from 4 week-old mouse testis was used as a positive control (+), and RNA from 4 week-old ovary without reverse transcription
(RT) was used as a negative control (-).
The c-DNA synthesized by reverse transcription
was amplified by PCR.
Properties of the reverse transcription
reaction in mRNA quantification.
RNA Extraction and Strand-Specific Reverse Transcription
. After infection with NDV La Sota, the supernatant and cells were collected and thoroughly mixed and 500 [micro]L of the mixture was lysed with 1mL TRIZol reagent (Invitrogen, Carlsbad, CA, USA).
"Our study reveals that the virus actually enters the CD4 T cells that are destined to die and that the virus starts to make a DNA copy of its RNA, a process called reverse transcription
. However, this process does not work well in the majority of these cells and the incomplete DNA intermediates that accumulate in the cytoplasm are sensed and trigger the cells to 'commit suicide' in an attempt to protect the body," said Dr.
This edition has been updated to include newer technologies like multiplex and real-time quantitative RT-PCR, RT-LATE-PCR, and recent advances in creating the optimum RT-PCR reaction, like RNA extraction, primer design, and reverse transcription
. Materials and reagents, reproducible protocols, and troubleshooting and pitfalls advice are given by the group of scientists, who work in physiology, biophysics, microbiology, and other fields around the world.
The test employs real-time reverse transcription
(RT) polymerase chain reaction (PCR) to detect the RNA of the 2009 H1N1 flu virus (2009 H1N1) in a patient's nasal or nasopharyngeal specimens.
In vivo studies suggested the drug inhibited HIV replication at or before the reverse transcription
, transcriptional latency, AIDS.
was performed on 2 [micro]g RNA, in 20 [micro]L final volume.